METHOD FOR UNBIASED MRNA AMPLIFICATION

专利名称：METHOD FOR UNBIASED MRNA

AMPLIFICATION

发明人：WANG, Bruce,CHUNG, Alicia申请号：EP98957858.8申请日：19981112公开号：EP1032704A1公开日：20000906

摘要：Methods of enzymatically producing unbiased amounts of nucleic acid frommRNA are provided. In the subject methods, a sample of mRNA is converted to ds cDNAusing a primer containing an RNA polymerase site and at least one priming site. Theresultant ds cDNA is then asymmetrically amplified with captureable primer to producecaptureable cDNA which is then converted to captured ds cDNA. The resultant ds cDNAmay then be used in a number of different applications, such as in the preparation ofamplified amounts of aRNA, in the preparation of cDNA probes, and the like.

申请人：INCYTE PHARMACEUTICALS, INC.

地址：3174 Porter Drive Palo Alto, CA 94304 US

国籍：US

代理机构：Wright, Simon Mark

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